Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans.
Identifieur interne : 000261 ( Main/Exploration ); précédent : 000260; suivant : 000262Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans.
Auteurs : Abhishek Anand [Suisse] ; Delphine Chinchilla [Suisse] ; Christopher Tan [Suisse] ; Laurent Mène-Saffrané [Suisse] ; Floriane L'Haridon [Suisse] ; Laure Weisskopf [Suisse]Source :
- Microorganisms [ 2076-2607 ] ; 2020.
Abstract
Plants face many biotic and abiotic challenges in nature; one of them is attack by disease-causing microbes. Phytophthora infestans, the causal agent of late blight is one of the most prominent pathogens of the potato responsible for multi-billion-dollar losses every year. We have previously reported that potato-associated Pseudomonas strains inhibited P. infestans at various developmental stages. A comparative genomics approach identified several factors putatively involved in this anti-oomycete activity, among which was the production of hydrogen cyanide (HCN). Here, we report the relative contribution of HCN emission to the overall anti-Phytophthora activity of two cyanogenic Pseudomonas strains, P. putida R32 and P. chlororaphis R47. To quantify this contribution, we generated HCN-negative mutants (Δhcn) and compared their activities to those of their respective wild types in different experiments assessing P. infestans mycelial growth, zoospore germination, and infection of potato leaf disks. Using in vitro experiments allowing only volatile-mediated interactions, we observed that HCN accounted for most of the mycelial growth inhibition (57% in R47 and 80% in R32). However, when allowing both volatile and diffusible compound-mediated interactions, HCN only accounted for 1% (R47) and 18% (R32) of mycelial growth inhibition. Likewise, both mutants inhibited zoospore germination in a similar way as their respective wild types. More importantly, leaf disk experiments showed that both wild-type and Δhcn strains of R47 and R32 were able to limit P. infestans infection to a similar extent. Our results suggest that while HCN is a major contributor to the in vitro volatile-mediated restriction of P. infestans mycelial growth, it does not play a major role in the inhibition of other disease-related features such as zoospore germination or infection of plant tissues.
DOI: 10.3390/microorganisms8081144
PubMed: 32731625
PubMed Central: PMC7464445
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Contribution of Hydrogen Cyanide to the Antagonistic Activity of <i>Pseudomonas</i> Strains Against <i>Phytophthora infestans</i>.</title><author><name sortKey="Anand, Abhishek" sort="Anand, Abhishek" uniqKey="Anand A" first="Abhishek" last="Anand">Abhishek Anand</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Chinchilla, Delphine" sort="Chinchilla, Delphine" uniqKey="Chinchilla D" first="Delphine" last="Chinchilla">Delphine Chinchilla</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Tan, Christopher" sort="Tan, Christopher" uniqKey="Tan C" first="Christopher" last="Tan">Christopher Tan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Mene Saffrane, Laurent" sort="Mene Saffrane, Laurent" uniqKey="Mene Saffrane L" first="Laurent" last="Mène-Saffrané">Laurent Mène-Saffrané</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="L Haridon, Floriane" sort="L Haridon, Floriane" uniqKey="L Haridon F" first="Floriane" last="L'Haridon">Floriane L'Haridon</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Weisskopf, Laure" sort="Weisskopf, Laure" uniqKey="Weisskopf L" first="Laure" last="Weisskopf">Laure Weisskopf</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32731625</idno><idno type="pmid">32731625</idno><idno type="doi">10.3390/microorganisms8081144</idno><idno type="pmc">PMC7464445</idno><idno type="wicri:Area/Main/Corpus">000115</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000115</idno><idno type="wicri:Area/Main/Curation">000115</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000115</idno><idno type="wicri:Area/Main/Exploration">000115</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Contribution of Hydrogen Cyanide to the Antagonistic Activity of <i>Pseudomonas</i> Strains Against <i>Phytophthora infestans</i>.</title><author><name sortKey="Anand, Abhishek" sort="Anand, Abhishek" uniqKey="Anand A" first="Abhishek" last="Anand">Abhishek Anand</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Chinchilla, Delphine" sort="Chinchilla, Delphine" uniqKey="Chinchilla D" first="Delphine" last="Chinchilla">Delphine Chinchilla</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Tan, Christopher" sort="Tan, Christopher" uniqKey="Tan C" first="Christopher" last="Tan">Christopher Tan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Mene Saffrane, Laurent" sort="Mene Saffrane, Laurent" uniqKey="Mene Saffrane L" first="Laurent" last="Mène-Saffrané">Laurent Mène-Saffrané</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="L Haridon, Floriane" sort="L Haridon, Floriane" uniqKey="L Haridon F" first="Floriane" last="L'Haridon">Floriane L'Haridon</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author><author><name sortKey="Weisskopf, Laure" sort="Weisskopf, Laure" uniqKey="Weisskopf L" first="Laure" last="Weisskopf">Laure Weisskopf</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Biology, University of Fribourg, 1700 Fribourg</wicri:regionArea><orgName type="university">Université de Fribourg</orgName><placeName><settlement type="city">Fribourg</settlement><region nuts="3" type="region">Canton de Fribourg</region></placeName></affiliation></author></analytic><series><title level="j">Microorganisms</title><idno type="ISSN">2076-2607</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants face many biotic and abiotic challenges in nature; one of them is attack by disease-causing microbes. <i>Phytophthora infestans</i>, the causal agent of late blight is one of the most prominent pathogens of the potato responsible for multi-billion-dollar losses every year. We have previously reported that potato-associated <i>Pseudomonas</i> strains inhibited <i>P. infestans</i> at various developmental stages. A comparative genomics approach identified several factors putatively involved in this anti-oomycete activity, among which was the production of hydrogen cyanide (HCN). Here, we report the relative contribution of HCN emission to the overall anti-<i>Phytophthora</i> activity of two cyanogenic <i>Pseudomonas</i> strains, <i>P. putida</i> R32 and <i>P. chlororaphis</i> R47. To quantify this contribution, we generated HCN-negative mutants (Δhcn) and compared their activities to those of their respective wild types in different experiments assessing <i>P. infestans</i> mycelial growth, zoospore germination, and infection of potato leaf disks. Using in vitro experiments allowing only volatile-mediated interactions, we observed that HCN accounted for most of the mycelial growth inhibition (57% in R47 and 80% in R32). However, when allowing both volatile and diffusible compound-mediated interactions, HCN only accounted for 1% (R47) and 18% (R32) of mycelial growth inhibition. Likewise, both mutants inhibited zoospore germination in a similar way as their respective wild types. More importantly, leaf disk experiments showed that both wild-type and Δhcn strains of R47 and R32 were able to limit <i>P. infestans</i> infection to a similar extent. Our results suggest that while HCN is a major contributor to the in vitro volatile-mediated restriction of <i>P. infestans</i> mycelial growth, it does not play a major role in the inhibition of other disease-related features such as zoospore germination or infection of plant tissues.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32731625</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-2607</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>8</Issue><PubDate><Year>2020</Year><Month>Jul</Month><Day>28</Day></PubDate></JournalIssue><Title>Microorganisms</Title><ISOAbbreviation>Microorganisms</ISOAbbreviation></Journal><ArticleTitle>Contribution of Hydrogen Cyanide to the Antagonistic Activity of <i>Pseudomonas</i> Strains Against <i>Phytophthora infestans</i>.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1144</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/microorganisms8081144</ELocationID><Abstract><AbstractText>Plants face many biotic and abiotic challenges in nature; one of them is attack by disease-causing microbes. <i>Phytophthora infestans</i>, the causal agent of late blight is one of the most prominent pathogens of the potato responsible for multi-billion-dollar losses every year. We have previously reported that potato-associated <i>Pseudomonas</i> strains inhibited <i>P. infestans</i> at various developmental stages. A comparative genomics approach identified several factors putatively involved in this anti-oomycete activity, among which was the production of hydrogen cyanide (HCN). Here, we report the relative contribution of HCN emission to the overall anti-<i>Phytophthora</i> activity of two cyanogenic <i>Pseudomonas</i> strains, <i>P. putida</i> R32 and <i>P. chlororaphis</i> R47. To quantify this contribution, we generated HCN-negative mutants (Δhcn) and compared their activities to those of their respective wild types in different experiments assessing <i>P. infestans</i> mycelial growth, zoospore germination, and infection of potato leaf disks. Using in vitro experiments allowing only volatile-mediated interactions, we observed that HCN accounted for most of the mycelial growth inhibition (57% in R47 and 80% in R32). However, when allowing both volatile and diffusible compound-mediated interactions, HCN only accounted for 1% (R47) and 18% (R32) of mycelial growth inhibition. Likewise, both mutants inhibited zoospore germination in a similar way as their respective wild types. More importantly, leaf disk experiments showed that both wild-type and Δhcn strains of R47 and R32 were able to limit <i>P. infestans</i> infection to a similar extent. Our results suggest that while HCN is a major contributor to the in vitro volatile-mediated restriction of <i>P. infestans</i> mycelial growth, it does not play a major role in the inhibition of other disease-related features such as zoospore germination or infection of plant tissues.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Anand</LastName><ForeName>Abhishek</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0001-8055-6970</Identifier><AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chinchilla</LastName><ForeName>Delphine</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Christopher</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mène-Saffrané</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>L'Haridon</LastName><ForeName>Floriane</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weisskopf</LastName><ForeName>Laure</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31003A_179310; 316030_177093</GrantID><Agency>Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Microorganisms</MedlineTA><NlmUniqueID>101625893</NlmUniqueID><ISSNLinking>2076-2607</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">biocontrol</Keyword><Keyword MajorTopicYN="N">hydrogen cyanide</Keyword><Keyword MajorTopicYN="N">potato late blight</Keyword><Keyword MajorTopicYN="N">pseudomonads</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>1</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32731625</ArticleId><ArticleId IdType="pii">microorganisms8081144</ArticleId><ArticleId IdType="doi">10.3390/microorganisms8081144</ArticleId><ArticleId IdType="pmc">PMC7464445</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Can J Microbiol. 2019 Mar;65(3):185-190</Citation><ArticleIdList><ArticleId IdType="pubmed">30398901</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2008 May;9(3):385-402</Citation><ArticleIdList><ArticleId IdType="pubmed">18705878</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2011 Oct;13(10):2702-16</Citation><ArticleIdList><ArticleId IdType="pubmed">21883790</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2017 Mar;107(3):273-279</Citation><ArticleIdList><ArticleId IdType="pubmed">27827009</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1998 Jun;180(12):3187-96</Citation><ArticleIdList><ArticleId IdType="pubmed">9620970</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2007 Jan;73(2):390-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17098912</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2016 Aug 4;54:529-47</Citation><ArticleIdList><ArticleId IdType="pubmed">27359366</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Pathol J. 2018 Feb;34(1):35-43</Citation><ArticleIdList><ArticleId IdType="pubmed">29422786</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 1997 May;24(3):579-91</Citation><ArticleIdList><ArticleId IdType="pubmed">9179851</ArticleId></ArticleIdList></Reference><Reference><Citation>J Appl Microbiol. 2015 Feb;118(2):267-74</Citation><ArticleIdList><ArticleId IdType="pubmed">25410133</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2015 Feb;81(3):821-30</Citation><ArticleIdList><ArticleId IdType="pubmed">25398872</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2019 Dec 30;9(1):18778</Citation><ArticleIdList><ArticleId IdType="pubmed">31889050</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2020 Apr 30;11:857</Citation><ArticleIdList><ArticleId IdType="pubmed">32425922</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2005;43:171-90</Citation><ArticleIdList><ArticleId IdType="pubmed">16078881</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2015 Nov 23;6:1295</Citation><ArticleIdList><ArticleId IdType="pubmed">26635763</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2018 Oct 29;9:2573</Citation><ArticleIdList><ArticleId IdType="pubmed">30420845</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2015 May 01;6:398</Citation><ArticleIdList><ArticleId IdType="pubmed">25983730</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2005 Apr;3(4):307-19</Citation><ArticleIdList><ArticleId IdType="pubmed">15759041</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol Rep. 2014 Feb;6(1):28-34</Citation><ArticleIdList><ArticleId IdType="pubmed">24596260</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2001 Jun;12(3):289-95</Citation><ArticleIdList><ArticleId IdType="pubmed">11404107</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2014 Oct;32(10):493-6</Citation><ArticleIdList><ArticleId IdType="pubmed">25246168</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):3469-3472</Citation><ArticleIdList><ArticleId IdType="pubmed">28289203</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2015 Nov 27;6:1309</Citation><ArticleIdList><ArticleId IdType="pubmed">26640460</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2019 Sep;109(9):1555-1565</Citation><ArticleIdList><ArticleId IdType="pubmed">31041882</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2012 Oct;96(1):37-48</Citation><ArticleIdList><ArticleId IdType="pubmed">22864968</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2017 Feb 03;8:100</Citation><ArticleIdList><ArticleId IdType="pubmed">28217113</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Fribourg</li></region><settlement><li>Fribourg</li></settlement><orgName><li>Université de Fribourg</li></orgName></list><tree><country name="Suisse"><region name="Canton de Fribourg"><name sortKey="Anand, Abhishek" sort="Anand, Abhishek" uniqKey="Anand A" first="Abhishek" last="Anand">Abhishek Anand</name></region><name sortKey="Chinchilla, Delphine" sort="Chinchilla, Delphine" uniqKey="Chinchilla D" first="Delphine" last="Chinchilla">Delphine Chinchilla</name><name sortKey="L Haridon, Floriane" sort="L Haridon, Floriane" uniqKey="L Haridon F" first="Floriane" last="L'Haridon">Floriane L'Haridon</name><name sortKey="Mene Saffrane, Laurent" sort="Mene Saffrane, Laurent" uniqKey="Mene Saffrane L" first="Laurent" last="Mène-Saffrané">Laurent Mène-Saffrané</name><name sortKey="Tan, Christopher" sort="Tan, Christopher" uniqKey="Tan C" first="Christopher" last="Tan">Christopher Tan</name><name sortKey="Weisskopf, Laure" sort="Weisskopf, Laure" uniqKey="Weisskopf L" first="Laure" last="Weisskopf">Laure Weisskopf</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000261 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000261 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32731625
|texte= Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32731625" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |